Two-Dimensional Crystallization on Lipid Monolayers and Three-Dimensional Structure of Sticholysin II, a Cytolysin from the Sea Anemone Stichodactyla helianthus
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چکیده
منابع مشابه
Crystallization and preliminary X-ray diffraction studies of the water-soluble state of the pore-forming toxin sticholysin II from the sea anemone Stichodactyla helianthus.
Sticholysin II (StnII) is a potent cytolytic protein produced by the sea anemone Stichodactyla helianthus. StnII belongs to the actinoporin family, a group of proteins which are characterized by their ability to spontaneously interact with biological membranes. The cytolytic character of these proteins is currently explained in terms of a molecular mechanism involving the formation of transmemb...
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An immobilized biocatalyst (ShIE-Octyl) was obtained by interfacial adsorption on Octyl-Sepharose CL 4B support of all interfacial esterases from the aqueous extract of the sea anemone Stichodactyla helianthus. ShIE-Octyl, synthesized by this simple method, contains semipurified interfacial esterases, including the isotoxins StI and StII. The immobilized esterases are maximally stable at pH 7.0...
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The major protease inhibitor from the sea anemone Stichodactyla helianthus (ShPI-1) is a non-specific inhibitor that binds trypsin and other trypsin-like enzymes, as well as chymotrypsin, and human neutrophil elastase. We performed site-directed mutagenesis of ShPI-1 to produce two variants (rShPI-1/K13L and rShPI/Y15S) that were expressed in Pichia pastoris, purified, and characterized. After ...
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متن کاملA Three- Dimensional Model of the Caspian Sea
Observations of the Caspian Sea during August-September 1995 are used to develop a three-dimensional numerical model to be used in calculating temperature and current. The model has variable grid resolution and horizontal smoothing that filters out small scale vertical motion. Data from the meteorological buoy network on the Caspian Sea are combined with routine observations at first-order syno...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2000
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(00)76855-x